Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR

Andréia Spanamberg; Ana Paula Ravazzolo; Ricardo Araujo; Natália Franceschi; Laerte Ferreiro

doi:10.1016/j.mmcr.2023.01.002

Bovine ringworm - Detection of Trichophyton verrucosum by SYBR-Green real-time PCR

Med Mycol Case Rep. 2023 Jan 31:39:34-37. doi: 10.1016/j.mmcr.2023.01.002. eCollection 2023 Mar.

Authors

Andréia Spanamberg^{1

2}, Ana Paula Ravazzolo³, Ricardo Araujo^{4

5}, Natália Franceschi^{1

2}, Laerte Ferreiro^{1

2}

Affiliations

¹ Setor de Micologia Veterinária, Departamento de Patologia Clínica Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
² Programa de Pós-graduação em Ciências Veterinárias (PPGCV), UFRGS, Porto Alegre, RS, Brazil.
³ Laboratório de Imunologia e Biologia Molecular, Faculdade de Veterinária, UFRGS, Brazil.
⁴ i3S, Instituto de Investigação e Inovação em Saúde, Porto, Portugal.
⁵ INEB - Instituto Nacional de Engenharia Biomédica, Universidade do Porto, Portugal.

Abstract

Trichophyton verrucosum is the most commonly dermatophyte involved in cattle ringworm. This work reported a case of bovine dermatophytosis due to Trichophyton verrucosum detected from the clinical sample by SYBR-Green real-time PCR. The strategy was based on the DNA extraction directly from the infected hair followed by real-time PCR and melting-point analysis. A faster and differential diagnosis was observed when compared to the conventional mycological methodology for detection and identification of Trichophyton verrucosum.

Keywords: Cattle; Dermatophytes; Hair; Ringworm; Trichophyton verrucosum.

Publication types

Case Reports