Characterization of photosynthetic Bradyrhizobium sp. strain SSBR45 isolated from the root nodules of Aeschynomene indica

Shingo Hata; Serina Kojima; Risa Tsuda; Nanami Kawajiri; Hiroshi Kouchi; Takamasa Suzuki; Kazuma Uesaka; Aiko Tanaka

doi:10.1080/15592324.2023.2184907

Characterization of photosynthetic Bradyrhizobium sp. strain SSBR45 isolated from the root nodules of Aeschynomene indica

Plant Signal Behav. 2023 Dec 31;18(1):2184907. doi: 10.1080/15592324.2023.2184907.

Authors

Shingo Hata¹, Serina Kojima¹, Risa Tsuda¹, Nanami Kawajiri¹, Hiroshi Kouchi², Takamasa Suzuki³, Kazuma Uesaka⁴, Aiko Tanaka⁵

Affiliations

¹ Faculty of Agriculture, Ryukoku University, Otsu, Japan.
² Division of Arts and Sciences, International Christian University, Mitaka, Japan.
³ College of Bioscience and Biotechnology, Chubu University, Kasugai, Japan.
⁴ Center for Gene Research, Nagoya University, Nagoya, Japan.
⁵ Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.

Abstract

We isolated a novel strain of Bradyrhizobium sp., SSBR45, from the nodulated roots of Aeschynomene indica and labeled it with Discosoma sp. red fluorescent protein (dsRED) or enhanced green fluorescent protein (eGFP) and determined its draft genomic sequence. The labeled SSBR45 stimulated the growth of A. indica markedly on a nitrogen-free medium, as observed by visualizing the fluorescent root nodules. The nodulated roots also exhibited high acetylene reduction activities. The SSBR45 genome included genes involved in nitrogen fixation, photosynthesis, and type IV secretion system; however, it did not consist of canonical nodABC genes and type III secretion system genes. SSBR45, a novel species of the genus Bradyrhizobium, consisted of an average nucleotide identity and average amino acid identity of 87% and 90%, respectively, with the closest strain B. oligotrophicum S58.

Keywords: Aeschynomene indica; Bradyrhizobium; fluorescence; genome; root nodule.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetylene
Amino Acids
Bradyrhizobium* / genetics
Fabaceae*
Photosynthesis / genetics

Substances

Acetylene
Amino Acids

Grants and funding

The present research was partly supported by grants from the Research Institute for Food and Agriculture, Ryukoku University to SH, and JSPS Grant-in-Aid for Scientific Research (C) (22K06078) to AT.