Peptidomics: LC-MS operational parameters do matter

Amélie Descamps; Kevin Van der Borght; Anton De Spiegeleer; Evelien Wynendaele; Bart De Spiegeleer

doi:10.1016/j.jpba.2023.115348

Peptidomics: LC-MS operational parameters do matter

J Pharm Biomed Anal. 2023 May 30:229:115348. doi: 10.1016/j.jpba.2023.115348. Epub 2023 Mar 20.

Authors

Amélie Descamps¹, Kevin Van der Borght², Anton De Spiegeleer³, Evelien Wynendaele¹, Bart De Spiegeleer⁴

Affiliations

¹ Drug Quality and Registration (DruQuaR) group, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Translational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) group, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium.
² Drug Quality and Registration (DruQuaR) group, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
³ Drug Quality and Registration (DruQuaR) group, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Translational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) group, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium; Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University, Corneel Heymanslaan 10, 9000 Ghent, Belgium.
⁴ Drug Quality and Registration (DruQuaR) group, Department of Pharmaceutical Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Translational Research in Immunosenescence, Gerontology and Geriatrics (TRIGG) group, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium. Electronic address: bart.despiegeleer@ugent.be.

PMID: 36963248
DOI: 10.1016/j.jpba.2023.115348

Abstract

The sensitive and specific detection of peptides at low levels in biofluids is critical to increase the lab-to-human translation of peptidomic research. An interesting group of peptides with increasing evidence for involvement in human diseases are quorum sensing peptides. To obtain more reliable conclusions on peptide measurands in biofluids, a selection of often neglected parts of the analytical process using LC-MS were investigated, with novel approaches recommended for each part. Quorum sensing peptides were used as the main model-peptides. The peptidomic parts investigated and discussed here are: Our work addresses aQbD-approached solutions to these challenges, encompassing sample stabilization measures, a suitable peptide anti-adsorption tool, judicious choice of injection solvent versus gradient system and optimal duty cycle parameters. Our recommendations will improve the peptidomics bio-analytics of not only quorum sensing peptides, but can also be of value for other measurands at low concentrations.

Keywords: AQbD; Bio-analysis; LC-MS; Microbiome; Peptidomics; Quorum Sensing Peptides.

Publication types

Review

MeSH terms

Chromatography, Liquid
Humans
Peptides*
Quorum Sensing
Tandem Mass Spectrometry*

Substances

Peptides