Investigation of Dengue Infection in Asymptomatic Individuals during a Recent Outbreak in La Réunion

Viruses. 2023 Mar 14;15(3):742. doi: 10.3390/v15030742.

Abstract

The number of dengue cases has increased dramatically over the past 20 years and is an important concern, particularly as the trends toward urbanization continue. While the majority of dengue cases are thought to be asymptomatic, it is unknown to what extent these contribute to transmission. A better understanding of their importance would help to guide control efforts. In 2019, a dengue outbreak in La Reunion resulted in more than 18,000 confirmed cases. Between October 2019 and August 2020, 19 clusters were investigated in the south, west, and east of the island, enabling the recruitment of 605 participants from 368 households within a 200 m radius of the home of the index cases (ICs). No active asymptomatic infections confirmed by RT-PCR were detected. Only 15% were possible asymptomatic dengue infections detected by the presence of anti-dengue IgM antibodies. Only 5.3% of the participants had a recent dengue infection confirmed by RT-PCR. Although the resurgence of dengue in La Réunion is very recent (2016), the rate of anti-dengue IgG positivity, a marker of past infections, was already high at 43% in this study. Dengue transmission was focal in time and space, as most cases were detected within a 100-m radius of the ICs, and within a time interval of less than 7 days between infections detected in a same cluster. No particular demographic or socio-cultural characteristics were associated with dengue infections. On the other hand, environmental risk factors such as type of housing or presence of rubbish in the streets were associated with dengue infections.

Keywords: La Réunion; asymptomatic infections; cluster study; dengue; dengue outbreak.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes*
  • Animals
  • Antibodies, Viral
  • Dengue Virus* / genetics
  • Disease Outbreaks
  • Humans
  • Reunion / epidemiology

Substances

  • Antibodies, Viral

Grants and funding

This research was funded mainly by the Swiss National Foundation for Scientific Research, grant number 179532. The first author, Olga DE SANTIS, was also funded by the GlobalP3HS program for Global Ph.D. Fellowship in Public Health Sciences, funded by Marie Sklodowska-Curie Actions (Horizon 2020–COFUND). INSERM Reacting contributed to the funding of the research. Part of the blood analyses was funded by the European Regional Development Fund through RUNDENG project number RE0022937.