In recent years, evidence showing metabolism as a fundamental regulator of stem cell functions has emerged. In skeletal muscle, its stem cells (satellite cells) sustain muscle regeneration, although they lose their regenerative potential with aging, and this has been attributed, at least in part, to changes in their metabolism. In this chapter, we describe a protocol to analyze the metabolism of satellite cells using the Seahorse technology, which can be applied to aging mice.
Keywords: Aging; Extracellular Acidification rate; Metabolism; Oxygen consumption rate; Satellite cell; Seahorse Technology; Skeletal muscle; Stem cells; Tissue regeneration.
© 2023. Springer Science+Business Media, LLC, part of Springer Nature.