Antimicrobial activity of antidepressants on normal gut microbiota: Results of the in vitro study

Grigory Rukavishnikov; Lubov Leonova; Evgeny Kasyanov; Vadim Leonov; Nikholay Neznanov; Galina Mazo

doi:10.3389/fnbeh.2023.1132127

Antimicrobial activity of antidepressants on normal gut microbiota: Results of the in vitro study

Front Behav Neurosci. 2023 Mar 22:17:1132127. doi: 10.3389/fnbeh.2023.1132127. eCollection 2023.

Authors

Grigory Rukavishnikov¹, Lubov Leonova¹, Evgeny Kasyanov¹, Vadim Leonov², Nikholay Neznanov^{3

4}, Galina Mazo¹

Affiliations

¹ Social Neuropsychiatry Department, Bekhterev National Medical Research Center for Psychiatry and Neurology, Saint-Petersburg, Russia.
² Department of Natural Sciences, Technology and Environmental Studies, Södertörn University, Stockholm, Sweden.
³ Geriatric Psychiatry Department, Bekhterev National Medical Research Center for Psychiatry and Neurology, Saint-Petersburg, Russia.
⁴ Psychiatry and Addictions Department, Pavlov First Saint-Petersburg State Medical University, Saint-Petersburg, Russia.

Abstract

Currently, there is little published data on the effects of antidepressants on normal gut microbiota and the consequences of such effects on treatment outcomes. The aim of the study: was to evaluate the growth kinetics of normal human gut microorganisms with antidepressants most common in routine clinical practice. Materials and methods: Research objects were species of microorganisms representing normal gut microbiota: Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Candida albicans ATCC 24433, Bifidobacterium 791, Enterococcus faecalis ATCC 29212, Lactobacillus rhamnosus ATCC 53103. All microorganisms were cultivated in Schaedler broth (HiMedia) under aerobic/anaerobic conditions. The active substances of all studied antidepressants (fluvoxamine, fluoxetine, escitalopram, duloxetine, venlafaxine, mirtazapine) were extracted from ground preparations by dimethyl sulfoxide and centrifuged. Each solution of antidepressants was added to a Schaedler broth containing a certain microorganism's strain and diluted to final concentrations-200 μg/ml, 500 μg/ml, and 700 μg/ml. For a quantitative assessment of the effect, the specific growth rates (μ, h-1) of microorganisms were calculated as the slope of the initial part of the growth curve in coordinates (lnA, t). To evaluate the antidepressant effects on representatives of the normal microbiota in vitro, the following parameters were chosen: specific growth rate and IC50. Results: All antidepressants had an inhibitory effect on the growth of all studied microorganisms. Fluvoxamine and venlafaxine had the least effect on the growth activity of all studied microorganisms. Fluoxetine showed a pronounced effect on growth activity against E. coli, E. feacalis, S. aureus, and the least effect against C. albicans. Escitalopram had a greater effect on the growth rate of E. coli, E. feacalis, B. bifidum, L. rhamnosus, and C. albicans, which puts it among the leaders in terms of its effect on the growth activity of the microorganisms we studied. Mirtazapine, according to the results of our experiment, showed the greatest activity against L. rhamnosus and C. albicans. Conclusions: Our results confirm the effects of antidepressants on the growth activity of the normal gut microbiota individual strains. Further study of the antimicrobial activity of antidepressants may become one of the new directions for optimizing the personalized therapy of patients with depression.

Keywords: antibacterial; antidepressants; depression; growth activity; microbiota-gut-brain axis.