The biology and type I/III hybrid nature of type I-D CRISPR-Cas systems

Tess M McBride; Shaharn C Cameron; Peter C Fineran; Robert D Fagerlund

doi:10.1042/BCJ20220073

The biology and type I/III hybrid nature of type I-D CRISPR-Cas systems

Biochem J. 2023 Apr 13;480(7):471-488. doi: 10.1042/BCJ20220073.

Authors

Tess M McBride^{1

2}, Shaharn C Cameron^{1

2

3}, Peter C Fineran^{1

2

3}, Robert D Fagerlund^{1

2

3}

Affiliations

¹ Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin 9054, New Zealand.
² Genetics Otago, University of Otago, Dunedin, New Zealand.
³ Bioprotection Aotearoa, University of Otago, PO Box 56, Dunedin 9054, New Zealand.

Abstract

Prokaryotes have adaptive defence mechanisms that protect them from mobile genetic elements and viral infection. One defence mechanism is called CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins). There are six different types of CRISPR-Cas systems and multiple subtypes that vary in composition and mode of action. Type I and III CRISPR-Cas systems utilise multi-protein complexes, which differ in structure, nucleic acid binding and cleaving preference. The type I-D system is a chimera of type I and III systems. Recently, there has been a burst of research on the type I-D CRISPR-Cas system. Here, we review the mechanism, evolution and biotechnological applications of the type I-D CRISPR-Cas system.

Keywords: CRISPR; RNA-binding proteins; bacteriophages; protein structure.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Biology
CRISPR-Associated Proteins* / genetics
CRISPR-Cas Systems*

Substances

CRISPR-Associated Proteins