The development of single cell approaches has facilitated the investigation of cellular heterogeneity and cell type-specific gene expression in complex tissues. Adipose tissue depots contain lipid storing adipocytes as well as a diverse array of cell types that form the adipocyte niche and regulate adipose tissue function. Here, I describe two protocols for the isolation of single cells and nuclei from white and brown adipose tissue. Additionally, I provide a detailed workflow for isolation of cell type- or lineage-specific single nuclei using nuclear tagging and translating ribosome affinity purification (NuTRAP) mouse models.
Keywords: Adipocyte progenitors; Adipose tissue; Transcriptomics; scRNA-seq; snRNA-seq.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.