Aggregation-Induced Electrochemiluminescence Based on a Zinc-Based Metal-Organic Framework and a Double Quencher Au@UiO-66-NH2 for the Sensitive Detection of Amyloid β 42 via Resonance Energy Transfer

Anal Chem. 2023 May 2;95(17):7045-7052. doi: 10.1021/acs.analchem.3c00729. Epub 2023 Apr 20.

Abstract

A novel sandwich electrochemiluminescence (ECL) biosensor based on aggregation-induced electrochemiluminescence resonance energy transfer (AIECL-RET) was designed for the sensitive detection of amyloid β42 (Aβ42). The synthesized silver nanoparticle-functionalized zinc metal-organic framework (Ag@ZnPTC) and gold nanoparticle-functionalized zirconium organic framework (Au@UiO-66-NH2) were used as the ECL donor and acceptor, respectively. AgNPs were generated in situ on the surface of ZnPTC, which further improved the ECL intensity and the loading of antibody 1 (Ab1). Under the optimized experimental conditions, the linear detection range of Aβ42 concentration was 10 fg/mL to 100 ng/mL, and the detection limit was 2.4 fg/mL (S/N = 3). The recoveries of Aβ42 were 99.5-104%. The method has good stability, repeatability, and specificity. Ag@ZnPTC/Au@UiO-66-NH2 provides an assay for the sensitive detection of disease biomarkers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides
  • Biosensing Techniques* / methods
  • Electrochemical Techniques / methods
  • Energy Transfer
  • Gold
  • Limit of Detection
  • Luminescent Measurements / methods
  • Metal Nanoparticles*
  • Metal-Organic Frameworks*
  • Organometallic Compounds*
  • Silver
  • Zinc

Substances

  • amyloid beta-protein (1-42)
  • UiO-66
  • Metal-Organic Frameworks
  • Amyloid beta-Peptides
  • Gold
  • Zinc
  • Silver
  • Organometallic Compounds