NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens

Yulia A Bogdanova; Elvira R Zaitseva; Alexander Yu Smirnov; Nadezhda S Baleeva; Alexey S Gavrikov; Ivan N Myasnyanko; Sergey A Goncharuk; Erik F Kot; Konstantin S Mineev; Alexander S Mishin; Mikhail S Baranov

doi:10.3390/ijms24097958

NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens

Int J Mol Sci. 2023 Apr 27;24(9):7958. doi: 10.3390/ijms24097958.

Authors

Yulia A Bogdanova¹, Elvira R Zaitseva¹, Alexander Yu Smirnov^{1

2}, Nadezhda S Baleeva^{1

2}, Alexey S Gavrikov¹, Ivan N Myasnyanko^{1

2}, Sergey A Goncharuk^{1

3}, Erik F Kot^{1

3}, Konstantin S Mineev^{1

3}, Alexander S Mishin¹, Mikhail S Baranov^{1

2}

Affiliations

¹ Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
² Laboratory of Medicinal Substances Chemistry, Institute of Translational Medicine, Pirogov Russian National Research Medical University, Ostrovitianov 1, 117997 Moscow, Russia.
³ Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia.

Abstract

In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in pairs with it in fluorescent microscopy as a genetically encoded tag.

Keywords: GFP; Kaede; bioluminescence; fluorescence; fluorogen; nanoLuc.

MeSH terms

Fluorescent Dyes* / metabolism
Luciferases / genetics
Microscopy, Fluorescence

Substances

nanoluc
Fluorescent Dyes
Luciferases

Grants and funding

18-73-10105/Russian Science Foundation