Phenotypic and genotypic features of POC1B-associated cone dystrophy

Ophthalmic Genet. 2024 Feb;45(1):72-77. doi: 10.1080/13816810.2023.2204361. Epub 2023 May 29.

Abstract

Purpose: Patients with cone dystrophy (CD) can present with virtually normal retinal appearance, which may delay diagnosis. This study describes the inconspicuous clinical features of POC1B-associated CD in two Saudi families.

Methods: This is a retrospective case study. Clinical data analyzed included multimodal retinal imaging and electroretinography of the affected individuals. Genetic analysis was done for all probands.

Results: Three affected males from two Saudi families with POC1B-associated CD were included. The ages at presentation ranged from 18 to 34 years. Ophthalmic examination showed decreased Snellen visual acuities (range: 20/100-20/300) and color vision bilaterally. Fundus examination showed only mild vascular attenuation. Macular optical coherence tomography showed reduced reflectivity of the external limiting membrane, ellipsoid, and interdigitation zones. Full-field electroretinography demonstrated undetectable light-adapted responses and normal dark-adapted responses in all patients. Next-generation sequencing showed one proband to be homozygous for a previously unpublished nonsense variant in POC1B (NM_172240):c.672C>G; p(Tyr224*). Whole exome sequencing for the second proband showed a novel homozygous frameshifting variant in POC1B: c.991del; p(Arg331Glufs*13).

Conclusion: We described two novel variants in POC1B and the associated subtle, yet significant retinal features. POC1B-associated CD is a rare cause of visual loss in patients with relatively normal fundus appearance. Deep phenotyping is necessary in formulating appropriate differential diagnosis.

Keywords: Cone dystrophy; POC1B gene; inherited retinal disease; normal fundus appearance; outer retinal atrophy.

MeSH terms

  • Cell Cycle Proteins / genetics
  • Cone Dystrophy* / diagnosis
  • Electroretinography
  • Fundus Oculi
  • Homozygote
  • Humans
  • Male
  • Mutation
  • Pedigree
  • Phenotype
  • Retrospective Studies
  • Tomography, Optical Coherence
  • Vision Disorders / diagnosis

Substances

  • POC1B protein, human
  • Cell Cycle Proteins