Retinyl Ester Analysis by Orbitrap Mass Spectrometry

Jeroen W A Jansen; Maya W Haaker; Esther A Zaal; J Bernd Helms

doi:10.1007/978-1-0716-3207-9_5

Retinyl Ester Analysis by Orbitrap Mass Spectrometry

Methods Mol Biol. 2023:2669:67-77. doi: 10.1007/978-1-0716-3207-9_5.

Authors

Jeroen W A Jansen¹, Maya W Haaker¹, Esther A Zaal¹, J Bernd Helms²

Affiliations

¹ Division of Cell Biology, Metabolism & Cancer, Faculty of Veterinary Medicine, Department of Biomolecular Health Sciences, Utrecht University, Utrecht, The Netherlands.
² Division of Cell Biology, Metabolism & Cancer, Faculty of Veterinary Medicine, Department of Biomolecular Health Sciences, Utrecht University, Utrecht, The Netherlands. J.B.Helms@uu.nl.

PMID: 37247055
DOI: 10.1007/978-1-0716-3207-9_5

Abstract

Retinoids are light-sensitive molecules that are normally detected by UV absorption techniques. Here we describe the identification and quantification of retinyl ester species by high-resolution mass spectrometry. Retinyl esters are extracted by the method of Bligh and Dyer and subsequently separated by HPLC in runs of 40 min. The retinyl esters are identified and quantified by mass spectrometry analysis. This procedure enables the highly sensitive detection and characterization of retinyl esters in biological samples such as hepatic stellate cells.

Keywords: Atmospheric pressure chemical ionization; High-performance liquid chromatography; Mass spectrometry; Orbitrap; Retinol; Retinyl esters; Vitamin A.

MeSH terms

Chromatography, High Pressure Liquid / methods
Mass Spectrometry / methods
Retinoids / analysis
Retinyl Esters* / analysis
Vitamin A*

Substances

Retinyl Esters
Vitamin A
Retinoids