SAIF: Label-Free Separation of Circulating Tumor Cells Using a Self-Amplified Inertial Focusing Microfluidic Chip

Aynur Abdulla; Xianting Ding

doi:10.1007/978-1-0716-3271-0_14

SAIF: Label-Free Separation of Circulating Tumor Cells Using a Self-Amplified Inertial Focusing Microfluidic Chip

Methods Mol Biol. 2023:2679:207-218. doi: 10.1007/978-1-0716-3271-0_14.

Authors

Aynur Abdulla¹, Xianting Ding²

Affiliations

¹ State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
² State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China. dingxianting@sjtu.edu.cn.

PMID: 37300618
DOI: 10.1007/978-1-0716-3271-0_14

Abstract

Circulating tumor cells (CTCs) are rare cells existing in the bloodstream with a relatively low number, which facilitate as a predictor of cancer progression. However, it is difficult to obtain highly purified intact CTCs with desired viability due to their low percentage among blood cells. In this chapter, we demonstrate the detailed steps for the fabrication and application of the novel self-amplified inertial-focused (SAIF) microfluidic chip that enables size-based, high-throughput, label-free separation of CTCs from the patient blood. The SAIF chip introduced in this chapter demonstrates the feasibility of an extremely narrow zigzag channel (with 40 μm channel width) connected with two expansion regions to effectively separate different-sized cells with amplified separation distance.

Keywords: Circulating tumor cells (CTCs); Inertial focusing; Label-free; Size based.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Separation
Humans
Microfluidic Analytical Techniques*
Microfluidics
Neoplastic Cells, Circulating* / pathology