From waste to the gut: Can blackcurrant press cake be a new functional ingredient? Insights on in vivo microbiota modulation, oxidative stress, and inflammation

Amanda Dos Santos Lima; Rômulo Dias Novaes; Lucas Cezar Pinheiro; Leonardo Augusto de Almeida; Hercia Stampini Duarte Martino; Alexandre Giusti-Paiva; Nora Pap; Daniel Granato; Luciana Azevedo

doi:10.1016/j.foodres.2023.112917

From waste to the gut: Can blackcurrant press cake be a new functional ingredient? Insights on in vivo microbiota modulation, oxidative stress, and inflammation

Food Res Int. 2023 Aug:170:112917. doi: 10.1016/j.foodres.2023.112917. Epub 2023 May 15.

Authors

Amanda Dos Santos Lima¹, Rômulo Dias Novaes², Lucas Cezar Pinheiro³, Leonardo Augusto de Almeida⁴, Hercia Stampini Duarte Martino⁵, Alexandre Giusti-Paiva⁶, Nora Pap⁷, Daniel Granato⁸, Luciana Azevedo⁹

Affiliations

¹ Faculty of Nutrition, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
² Institute of Biomedical Sciences, Department of Structural Biology, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
³ Department of Pharmacology, Federal University Santa Catarina, Santa Catarina, Brazil; Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
⁴ Department of Microbiology and Immunology, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
⁵ Department of Nutrition and Health, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
⁶ Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.
⁷ Biorefinery and Bioproducts, Production Systems Unit, Natural Resources Institute Finland (Luke), Myllytie 1, 31600 Jokioinen, Finland.
⁸ Bioactivity & Applications Lab, Department of Biological Sciences, Faculty of Science and Engineering, School of Natural Sciences, University of Limerick, V94 T9PX Limerick, Ireland. Electronic address: Daniel.Granato@ul.ie.
⁹ Faculty of Nutrition, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil. Electronic address: luciana.azevedo@unifal-mg.edu.br.

PMID: 37316039
DOI: 10.1016/j.foodres.2023.112917

Abstract

Blackcurrant press cake (BPC) is a source of anthocyanins, and this study evaluated the bioactivity and gut microbiota modulation of blackcurrant diets with or without 1,2 dimethylhydrazine (DMH)-induced colon carcinogenesis in rats. In colon cancer-induced rats (CRC), BPC at the highest dosages increased pro-inflammatory parameters and the expression of anti-apoptotic cytokines, accentuating colon cancer initiation by aberrant crypts and morphological changes. Fecal microbiome analysis showed that BPC altered the composition and function of the gut microbiome. This evidence suggests that high doses of BPC act as a pro-oxidant, accentuating the inflammatory environment and CRC progression.

Keywords: Aberrant crypt foci (ACF); Anthocyanins; Blackcurrant; By-product; Colorectal carcinogenesis; Gut microbiota modulation; Inflammation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anthocyanins / pharmacology
Colonic Neoplasms*
Inflammation
Microbiota*
Oxidative Stress
Pharmaceutical Vehicles
Rats

Substances

Anthocyanins
Pharmaceutical Vehicles