Modulation of cytokine release from peripheral blood mononuclear cells from multiple sclerosis patients by coenzyme A and soraphen A

Carolin Blask; Juliane Schulze; Sarah Rümpel; Marie Süße; Matthias Grothe; Stefan Gross; Alexander Dressel; Rolf Müller; Johanna Ruhnau; Antje Vogelgesang

doi:10.1016/j.jneuroim.2023.578135

Modulation of cytokine release from peripheral blood mononuclear cells from multiple sclerosis patients by coenzyme A and soraphen A

J Neuroimmunol. 2023 Aug 15:381:578135. doi: 10.1016/j.jneuroim.2023.578135. Epub 2023 Jun 21.

Authors

Affiliations

¹ Dept. of Neurology, University Medicine Greifswald, Germany.
² Dept. of Internal Medicine B, University Medicine Greifswald, Greifswald, Germany.
³ Dept. of Neurology, Carl-Thiem-Klinikum, Cottbus, Germany.
⁴ Department of Microbial Natural Products, Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI) and Department of Pharmacy, Saarland University, Campus E8 1, 66123 Saarbrücken, Germany.
⁵ Dept. of Neurology, University Medicine Greifswald, Germany. Electronic address: johanna.ruhnau@uni-greifswald.de.
⁶ Dept. of Neurology, University Medicine Greifswald, Germany. Electronic address: antje.vogelgesang@uni-greifswald.de.

PMID: 37364515
DOI: 10.1016/j.jneuroim.2023.578135

Abstract

By applying the acetyl-CoA-carboxylase inhibitors soraphen A (SorA) and coenzyme A (CoA) ex vivo, we aimed to reduce proinflammatory cytokine release by PBMCs and increase anti-inflammatory cytokine levels, thereby demonstrating a possible application of those pathways in future multiple sclerosis (MS) therapy. In a prospective exploratory monocentric study, we analysed cytokine production by PBMCs treated with SorA (10 or 50 nM) and CoA (600 μM). Thirty-one MS patients were compared to 18 healthy age-matched controls. We demonstrated the immunomodulatory potential of SorA and CoA in targeting the immune function of MS patients, with an overall reduction of cytokines except of IL-2, IL-6 and IL-10.

Keywords: Acetyl-CoA-carboxylase; Coenzyme A; Cytokines; Fatty acid metabolism; Multiple sclerosis; Soraphen A; Th17 cells; Treg.