In May 2021, an agricultural worker originally from Trementinal, Argentina, sought treatment for febrile illness in Tarija, Bolivia, where he resided at the time of illness onset. The patient tested negative for hantavirus RNA, but next-generation sequencing of a serum sample yielded a complete genome for Rio Negro virus.
Keywords: Bolivia; Rio Negro virus; arboviruses; febrile infections; vector-borne infections; viruses; zoonoses.