Effects of Graphene-Based Metal Composite and Urea on Seed Germination and Performance of Berberis chitria Buch.-Ham. ex Lindl

Appl Biochem Biotechnol. 2024 Apr;196(4):2219-2232. doi: 10.1007/s12010-023-04624-5. Epub 2023 Jul 25.

Abstract

Being an important source of berberine, Berberis chitria Buch.-Ham. ex Lindl. (Berberidaceae) has high demand in pharmaceutical industries. Its populations are diminishing due to overexploitation, habitat loss, slow-growing nature, and climate change. It is important to develop propagation protocols to sustain its natural populations and ensure its survival in the future. Fertilizers play an essential role in the yield and productivity of different crops. Among others, urea is the most abundantly used fertilizer in crops. Its effects on the yield and survival of medicinal plants are poorly studied. However, it is known that applying urea for a long time affects the soil negatively. Due to these negative effects, alternative fertilizers such as graphene-based metal composite (GMC) are being tested for their efficiency. In the present study, for the first time, we tested the effects of urea and GMC on the germination and performance of B. chitria. GMC showed maximum germination at 30 ppm (75%) and urea at 15 ppm (79%). Findings reveal non-significant effects of GMC and urea on germination and performance of B. chitria, suggesting the use of GMC as an alternative fertilizer.

Keywords: Berberine; Fertilizer; Graphene; Nanotechnology; Seed biology; Urea.

MeSH terms

  • Berberis* / chemistry
  • Berberis* / drug effects
  • Berberis* / growth & development
  • Fertilizers*
  • Germination* / drug effects
  • Graphite* / chemistry
  • Graphite* / pharmacology
  • Metals / chemistry
  • Metals / pharmacology
  • Seeds* / drug effects
  • Seeds* / growth & development
  • Urea* / chemistry
  • Urea* / pharmacology

Substances

  • Urea
  • Graphite
  • Fertilizers
  • Metals