Clinical, Immunological, and Genetic Findings in Iranian Patients with MHC-II Deficiency: Confirmation of c.162delG RFXANK Founder Mutation in the Iranian Population

J Clin Immunol. 2023 Nov;43(8):1941-1952. doi: 10.1007/s10875-023-01562-z. Epub 2023 Aug 16.

Abstract

Purpose: Major histocompatibility complex class II (MHC-II) deficiency is a rare inborn error of immunity (IEI). Impaired antigen presentation to CD4 + T cells results in combined immunodeficiency (CID). Patients typically present with severe respiratory and gastrointestinal tract infections at early ages. Hematopoietic stem cell transplantation (HSCT) is the only curative therapy.

Methods: We describe the clinical, immunologic, and genetic features of eighteen unrelated Iranian patients with MHC-II deficiency.

Results: Consanguinity was present in all affected families. The median age at the initial presentation was 5.5 months (range 7 days to 18 years). The main symptoms included failure to thrive, persistent diarrhea, and pneumonia. Autoimmune and neurologic features were also documented in about one-third of the patients, respectively. Thirteen patients carried RFXANK gene mutations, two carried RFX5 gene mutations, and three carried a RFXAP gene mutation. Six patients shared the same RFXANK founder mutation (c.162delG); limited to the Iranian population and dated to approximately 1296 years ago. Four of the patients underwent HSCT; three of them are alive. On the other hand, nine of the fourteen patients who did not undergo HSCT had a poor prognosis and died.

Conclusion: MHC-II deficiency is not rare in Iran, with a high rate of consanguinity. It should be considered in the differential diagnosis of CID at any age. With the limited access to HSCT and its variable results in MHC-II deficiency, implementing genetic counseling and family planning for the affected families are mandatory. We are better determined to study the c.162delG RFXANK heterozygous mutation frequency in the Iranian population.

Keywords: CD4 + lymphocytopenia; Founder effect; Hematopoietic stem cell transplantation; Inborn error of immunity; MHC-II deficiency; RFX5 gene; RFXANK gene; RFXAP gene.

MeSH terms

  • DNA-Binding Proteins* / genetics
  • Histocompatibility Antigens Class II / genetics
  • Humans
  • Infant, Newborn
  • Iran
  • Mutation / genetics
  • Severe Combined Immunodeficiency* / genetics
  • Transcription Factors* / genetics

Substances

  • DNA-Binding Proteins
  • Histocompatibility Antigens Class II
  • RFXANK protein, human
  • Transcription Factors