Updated Methods of Extracellular Vesicles Isolation

Hongyun Wang; Zijiang Yang; Songwei Ai; Junjie Xiao

doi:10.1007/978-981-99-1443-2_1

Updated Methods of Extracellular Vesicles Isolation

Adv Exp Med Biol. 2023:1418:3-14. doi: 10.1007/978-981-99-1443-2_1.

Authors

Hongyun Wang^{1

2}, Zijiang Yang², Songwei Ai¹, Junjie Xiao³

Affiliations

¹ Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Nantong, China.
² Cardiac Regeneration and Ageing Lab, Institute of Cardiovascular Sciences, School of Life Science, Shanghai University, Shanghai, China.
³ Shanghai Engineering Research Center of Organ Repair, School of Life Science, Shanghai University, Shanghai, China. junjiexiao@shu.edu.cn.

PMID: 37603269
DOI: 10.1007/978-981-99-1443-2_1

Abstract

Extracellular vesicles (EVs) are considered as cargo and mediate intercellular communication. As natural biological nanoparticles, EVs can be secreted by almost all kinds of cells and exist in biofluids such as milk, urine, blood, etc. In the past decades, several methods have been utilized to isolate EVs from cell culture medium, biofluids, and tissues. Here in this chapter, we summarized conventional and novel methods and fundamental procedures of EVs extraction and purification from different biofluids (plasma, urine, milk, and saliva) and tissues (brain, intestinal tissue, muscles, and heart). The present section also discusses how to choose appropriate methods to extract EVs from tissues based on downstream analysis. This chapter will expand the horizons of EVs isolation and purification from different mediums.

Keywords: Biofluids; Extracellular vesicles; Isolation; Tissues.

MeSH terms

Animals
Biological Transport
Body Fluids*
Brain
Extracellular Vesicles*
Milk