Anomeric preference of glucose phosphorylation and glycolysis in human erythrocytes

Biochem Int. 1986 Aug;13(2):359-65.

Abstract

Lactate output from the alpha and beta anomers of glucose was measured in intact human erythrocytes at 37 degrees C; and glucose anomer phosphorylation, in human erythrocyte homogenates. The rates of both glucose metabolism (lactate output) and phosphorylation were higher in the presence of beta-D-glucose as distinct from alpha-D-glucose at three glucose concentrations used (2, 5, and 10 mM). Thus, the v beta/v alpha ratios of metabolism and phosphorylation of glucose at 2 mM were 1.24 and 1.22, respectively. The results indicate that the beta preference of hexokinase, a rate-limiting enzyme in glycolysis, is reflected in beta-preferential glycolysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Glucose / metabolism*
  • Carbon Radioisotopes
  • Erythrocytes / metabolism*
  • Glycolysis*
  • Humans
  • Kinetics
  • Lactates / blood
  • Phosphorylation
  • Stereoisomerism

Substances

  • Blood Glucose
  • Carbon Radioisotopes
  • Lactates