Influence of legume-derived proteins with varying solubility on the direct expansion of corn starch during twin-screw extrusion processing

Breana Downs; Katarzyna Skrzypczak; Jana K Richter; Tomasz Krajka; Marina Ikuse; Joshua B Bernin; Girish M Ganjyal

doi:10.1111/1750-3841.16730

Influence of legume-derived proteins with varying solubility on the direct expansion of corn starch during twin-screw extrusion processing

J Food Sci. 2023 Oct;88(10):4169-4179. doi: 10.1111/1750-3841.16730. Epub 2023 Sep 15.

Authors

Breana Downs¹, Katarzyna Skrzypczak^{1

2}, Jana K Richter¹, Tomasz Krajka³, Marina Ikuse¹, Joshua B Bernin¹, Girish M Ganjyal¹

Affiliations

¹ School of Food Science, Washington State University, Pullman, Washington, USA.
² Sub-Department of Fruits, Vegetables and Mushrooms Technology, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Lublin, Poland.
³ Department of Production Computerisation and Robotisation, Lublin University of Technology, Lublin, Poland.

PMID: 37712742
DOI: 10.1111/1750-3841.16730

Abstract

This study analyzed the effect of the inclusion of legume-derived proteins, specifically pea and fava bean protein, with varying solubility levels on the expansion of corn starch. Three different proteins exhibiting low, medium, or high solubility were mixed with corn starch to obtain blends containing 15%, 25%, and 35% (w/w) of the protein. Extrusion was performed on a twin-screw extruder at three different screw speeds (200, 400, and 600 rpm), a moisture content of 16% (w.b.), and a die temperature of 140°C. Obtained extrudates were analyzed for their expansion, unit density, and hydration properties, namely, water solubility index (WSI) and water absorption index (WAI). Extrudates containing the protein with the highest solubility showed different patterns than those that had proteins with low or medium solubility. Expansion ratio (ER) increased from a maximum of 3.55 ± 0.24 for pure corn starch up to 5.45 ± 0.43 when incorporating 35% of the protein with medium solubility but significantly decreased down to 1.24 ± 0.08 when incorporating 35% of the most soluble protein. The influence on the system parameters, as well as on the hydration properties, was also greatest for the blends containing the protein with the highest solubility. Even though significant Pearson correlations were observed between protein solubility and ER (r = -0.579), unity density (r = 0.614), WSI (r = -0.634), torque (r = -0.612), as well as specific mechanical energy (r = -0.451), further research is needed to evaluate if the solubility is indeed the reason for certain behaviors or if other protein characteristics are more critical for expansion. PRACTICAL APPLICATION: This manuscript provides practical information on the influence of the addition of legume-derived proteins with different solubility levels on direct expansion. The obtained results may help the industry with the selection of the appropriate proteins for inclusion levels in producing high protein direct-expanded extruded food products.

Keywords: expansion; extrusion; legumes; protein solubility; starch.

Abstract

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