Molecular detection and internal transcribed spacer-1 sequence diversity of Trypanosoma evansi in goats from Cavite, Philippines

J Vet Med Sci. 2024 Jan 10;86(1):35-38. doi: 10.1292/jvms.23-0416. Epub 2023 Nov 23.

Abstract

Goat production is an important source of livelihood and food. Goats may serve as reservoir of surra affecting livestock production. Here, forty-two free-roaming goats from Cavite, Philippines were screened using two primer sets, Trypanosoma brucei minisatellite chromosome for initial detection and the internal transcribed spacer 1 (ITS-1) to determine phylogeny. Initial PCR detection showed that 19/42 (45%) goats were positive, much higher than the rate previously reported in goats from Cebu (34%). The infectivity rate was higher in male (56%) than in female (42%) and the rate was higher in young ≤1 year old (100%) than in adult >1 year old (43%). Phylogenetic analysis of the ITS-1 sequences between T. evansi goat samples and other isolates indicate potential interspecies transmission.

Keywords: Cavite; PCR; Trypanosoma evansi; goat; internal transcribed spacer-1.

MeSH terms

  • Animals
  • DNA, Protozoan / genetics
  • Female
  • Goat Diseases* / diagnosis
  • Goat Diseases* / epidemiology
  • Goats
  • Male
  • Philippines / epidemiology
  • Phylogeny
  • Trypanosoma* / genetics
  • Trypanosomiasis* / epidemiology
  • Trypanosomiasis* / veterinary

Substances

  • DNA, Protozoan