ALK1 Deficiency Impairs the Wound-Healing Process and Increases Mortality in Murine Model of Myocardial Infarction

J Cardiovasc Transl Res. 2024 Jun;17(3):496-504. doi: 10.1007/s12265-023-10471-w. Epub 2023 Dec 8.

Abstract

The functional role of TGFβ type I receptor, activin-like kinase (ALK)-1 in post-myocardial infarction (MI) cardiac remodeling is unknown. We hypothesize that reduced ALK1 activity reduces survival and promotes cardiac fibrosis after MI. MI was induced in wild-type (WT), and ALK+/- mice by left coronary ligation. After 14 days ALK1+/- mice had reduced survival with a higher rate of cardiac rupture compared to WT mice. ALK1+/- left ventricles (LVs) had increased volumes at the end of systole and at the end of diastole. After MI ALK1+/- LVs had increased profibrotic SMAD3 signaling, type 1 collagen, and fibrosis as well as increased levels of TGFβ1 co-receptor, endoglin, VEGF, and ALK1 ligands BMP9 and BMP10. ALK1+/- LVs had decreased levels of stromal-derived factor 1α. These data identify the critical role of ALK1 in post-MI survival and cardiac remodeling and implicate ALK1 as a potential therapeutic target to improve survival after MI.

Keywords: Activin-like kinase 1; Bone morphogenetic protein 9; Fibrosis; Myocardial infarction.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Activin Receptors, Type I / deficiency
  • Activin Receptors, Type I / genetics
  • Activin Receptors, Type I / metabolism
  • Activin Receptors, Type II* / genetics
  • Activin Receptors, Type II* / metabolism
  • Animals
  • Disease Models, Animal*
  • Fibrosis*
  • Male
  • Mice, Inbred C57BL*
  • Mice, Knockout*
  • Myocardial Infarction* / enzymology
  • Myocardial Infarction* / genetics
  • Myocardial Infarction* / metabolism
  • Myocardial Infarction* / pathology
  • Myocardium* / enzymology
  • Myocardium* / metabolism
  • Myocardium* / pathology
  • Signal Transduction*
  • Smad3 Protein / genetics
  • Smad3 Protein / metabolism
  • Time Factors
  • Ventricular Function, Left*
  • Ventricular Remodeling*

Substances

  • Acvrl1 protein, mouse
  • Activin Receptors, Type II
  • Smad3 Protein
  • Activin Receptors, Type I
  • Smad3 protein, mouse