Radiotherapy in the head and neck region influences the chemical and mechanical properties of intraradicular dentin

Alessandro Marcelo Pelloso; Matheus Elias Rossi; Rafael Resende de Miranda; Carlos José Soares; Veridiana Resende Novais

doi:10.1016/j.archoralbio.2023.105868

Radiotherapy in the head and neck region influences the chemical and mechanical properties of intraradicular dentin

Arch Oral Biol. 2024 Feb:158:105868. doi: 10.1016/j.archoralbio.2023.105868. Epub 2023 Dec 1.

Authors

Alessandro Marcelo Pelloso¹, Matheus Elias Rossi¹, Rafael Resende de Miranda¹, Carlos José Soares¹, Veridiana Resende Novais²

Affiliations

¹ Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Av Pará 1720, Block 4L, Campus Umuarama, Uberlândia, MG 38405-320, Brazil.
² Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Av Pará 1720, Block 4L, Campus Umuarama, Uberlândia, MG 38405-320, Brazil. Electronic address: veridianaresende@hotmail.com.

PMID: 38070323
DOI: 10.1016/j.archoralbio.2023.105868

Abstract

Objectives: To investigate the chemical and mechanical properties of intraradicular dentin submitted to radiotherapy.

Materials and methods: Sixteen mandibular incisors were divided into two groups (n = 8): non-irradiated and irradiated. The irradiated teeth were obtained from head and neck radiotherapy patients, with a total dose ranging from 70.2 to 72 Gy divided into 1.8 Gy daily. After sample preparation, intraradicular dentin slices of each root third were evaluated by Raman spectroscopy, energy dispersive spectroscopy and Knoop microhardness test. Data were analyzed by Two-way ANOVA and Tukey's test (α = 0.05).

Results: In Raman spectroscopy, carbonate and amide III showed a significant difference for irradiation and third (carbonate p = 0.021 and p < 0.001; amide III p < 0.001 and p = 0.001, respectively). For amide I, there was a significant difference for third (p < 0.001). For carbonate/mineral ratio, there was a significant difference for irradiation (p = 0.0016) and third (p < 0.001), with the irradiated middle third showing the lowest values. For amide I/amide III ratio, there was a significant difference for irradiation (p = 0.005) in the cervical third. In energy dispersive spectroscopy, carbon (p = 0.004; p = 0.020), phosphorus (p < 0.001; p = 0.009) and calcium (p = 0.008; p = 0.007) showed differences for irradiation and third, with the irradiated groups presenting lower values in cervical and middle thirds. For calcium/phosphorus ratio, there was a significant difference for irradiation (p < 0.001) in cervical and middle thirds. Regarding microhardness, there was a significant difference for irradiation (p < 0.001), with all irradiated groups showing lower microhardness values.

Conclusions: The radiotherapy altered the chemical and mechanical properties of intraradicular dentin, mainly in the cervical and middle root thirds.

Keywords: Chemical analysis; Dentin; Hardness; Radiotherapy.

MeSH terms

Amides / analysis
Calcium* / analysis
Carbonates / analysis
Dentin* / chemistry
Humans
Incisor
Materials Testing
Phosphorus / analysis

Substances

Calcium
Carbonates
Phosphorus
Amides