False aminoglycoside resistance in Enterobacterales and non-fermenters by an automated testing system: a descriptive study

Microbiol Spectr. 2023 Dec 12;11(6):e0309323. doi: 10.1128/spectrum.03093-23. Epub 2023 Nov 16.

Abstract

Antimicrobial sensitivity data are important to guide antimicrobial therapy. In microbiological laboratories, routine sensitivity measurements are typically performed with automated testing systems such as VITEK2 and Phoenix. Using data from the Dutch national surveillance system for antimicrobial resistance over a 6-year period, we found that the measured minimum inhibitory concentrations for aminoglycosides in Enterobacterales and non-fermenters were too high for the Phoenix system. In addition, we observed a yearly increase in resistance for several species measured by Phoenix. These findings might have consequences for clinical treatment of patients with sepsis.

Keywords: aminoglycosides; antimicrobial susceptibility testing; automated testing system; gentamicin.

MeSH terms

  • Aminoglycosides* / pharmacology
  • Anti-Bacterial Agents / pharmacology
  • Gammaproteobacteria*
  • Gram-Negative Bacteria
  • Humans
  • Laboratories

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents