A "chemical tongue" is proposed based on thiomalic acid-capped quantum dots (QDs) with signal enrichment provided by excitation-emission matrix (EEM) fluorescence spectroscopy for the determination of close structural analogs-short-length amyloid β (Aβ) peptides related to Alzheimer's disease. Excellent discrimination is obtained by principal component analysis (PCA) for seven derivatives: Aβ1-16, Aβ4-16, Aβ4-9, Aβ5-16, Aβ5-12, Aβ5-9, Aβ12-16. Detection of Aβ4-16, Aβ4-16, and Aβ5-9 in binary and ternary mixtures performed by QDs-based chemical tongue using partial least squares-discriminant analysis (PLS-DA) provided perfect 100% accuracy for the two studied peptides (Aβ4-16 and Aβ4-16), while for the third one (Aβ5-9) it was slightly lower (97.9%). Successful detection of Aβ4-16 at 1 pmol/mL (1.6 ng/mL) suggests that the detection limit of the proposed method for short-length Aβ peptides can span nanomolar concentrations. This result is highly promising for the development of simple and efficient methods for sequence recognition in short-length peptides and better understanding of mechanisms at the QD-analyte interface.
Keywords: 2D fluorescence; Alzheimer’s disease; Aβ peptides; Chemical tongue; Excitation-emission matrix fluorescence spectroscopy; Machine learning; Multispectral fluorescence spectroscopy; Peptide sequence recognition; Quantum dots; Short-length peptides.
© 2024. The Author(s).