Induction of Primordial Germ Cell-Like Cells from Rat Pluripotent Stem Cells

Mami Oikawa; Masumi Hirabayashi; Toshihiro Kobayashi

doi:10.1007/978-1-0716-3698-5_8

Induction of Primordial Germ Cell-Like Cells from Rat Pluripotent Stem Cells

Methods Mol Biol. 2024:2770:99-111. doi: 10.1007/978-1-0716-3698-5_8.

Authors

Mami Oikawa^{1

2}, Masumi Hirabayashi^{3

4}, Toshihiro Kobayashi^{5

6}

Affiliations

¹ Division of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
² Laboratory of Regenerative Medicine, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan.
³ Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Aichi, Japan.
⁴ The Graduate University of Advanced Studies, Aichi, Japan.
⁵ Division of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. tkoba@g.ecc.u-tokyo.ac.jp.
⁶ Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Aichi, Japan. tkoba@g.ecc.u-tokyo.ac.jp.

PMID: 38351449
DOI: 10.1007/978-1-0716-3698-5_8

Abstract

In vitro induction of primordial germ cell like-cells (PGCLCs) from pluripotent stem cells (PSCs) is a robust method that will contribute to understanding the fundamentals of cell fate decisions, animal breeding, and future reproductive medicine. Here, we introduce this system established in the rat model. We describe a stepwise protocol to induce epiblast-like cells and subsequent PGCLCs by forming spherical aggregates from rat PSCs. We also describe a protocol to mature these PGCLCs from specified/migratory to the gonadal stage by aggregation with female gonadal somatic cells.

Keywords: Cell aggregations; Epiblast-like cells; Gonadal somatic cells; Primordial germ cell-like cells; Rat pluripotent stem cells.

MeSH terms

Animals
Cell Differentiation
Cells, Cultured
Female
Germ Cells
Germ Layers
Pluripotent Stem Cells*
Rats