Dorsal motor vagal neurons can elicit bradycardia and reduce anxiety-like behavior

Abstract

Cardiovagal neurons (CVNs) innervate cardiac ganglia through the vagus nerve to control cardiac function. Although the cardioinhibitory role of CVNs in nucleus ambiguus (CVN^NA) is well established, the nature and functionality of CVNs in dorsal motor nucleus of the vagus (CVN^DMV) is less clear. We therefore aimed to characterize CVN^DMV anatomically, physiologically, and functionally. Optogenetically activating cholinergic DMV neurons resulted in robust bradycardia through peripheral muscarinic (parasympathetic) and nicotinic (ganglionic) acetylcholine receptors, but not beta-1-adrenergic (sympathetic) receptors. Retrograde tracing from the cardiac fat pad labeled CVN^NA and CVN^DMV through the vagus nerve. Using whole-cell patch-clamp, CVN^DMV demonstrated greater hyperexcitability and spontaneous action potential firing ex vivo despite similar resting membrane potentials, compared to CVN^NA. Chemogenetically activating DMV also caused significant bradycardia with a correlated reduction in anxiety-like behavior. Thus, DMV contains uniquely hyperexcitable CVNs and is capable of cardioinhibition and robust anxiolysis.

Keywords: Behavioral neuroscience; Biological sciences; Natural sciences; Neuroscience; Physiology; Systems neuroscience.