Characterization of Risk Factors for Modeling of Type 2 Diabetes Mellitus Induced by a High-Fat Diet in C57BL/6 Mice

N A Borozdina; E R Shaikhutdinova; G A Slashcheva; N A Goryacheva; A V Zamyatina; E S Sadovnikova; I A Pakhomova; V M Pavlov; N A Perepechenova; M S Severyukhina; A Yu Fedotova; D V Popkova; I N Gladkikh; E V Leichenko; I A Dyachenko

doi:10.1007/s10517-024-06047-6

Characterization of Risk Factors for Modeling of Type 2 Diabetes Mellitus Induced by a High-Fat Diet in C57BL/6 Mice

Bull Exp Biol Med. 2024 Feb;176(4):461-465. doi: 10.1007/s10517-024-06047-6. Epub 2024 Mar 15.

Authors

N A Borozdina^{1

2}, E R Shaikhutdinova³, G A Slashcheva³, N A Goryacheva^{3

4}, A V Zamyatina^{3

4}, E S Sadovnikova³, I A Pakhomova³, V M Pavlov^{3

4}, N A Perepechenova^{3

4}, M S Severyukhina^{3

4}, A Yu Fedotova^{3

4}, D V Popkova⁵, I N Gladkikh⁵, E V Leichenko⁵, I A Dyachenko^{3

4}

Affiliations

¹ Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow region, Russia. borozdina@bibch.ru.
² Pushchino Branch of Russian Biotechnological University (ROSBIOTECH), Pushchino, Moscow Region, Russia. borozdina@bibch.ru.
³ Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow region, Russia.
⁴ Pushchino Branch of Russian Biotechnological University (ROSBIOTECH), Pushchino, Moscow Region, Russia.
⁵ G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia.

PMID: 38491257
DOI: 10.1007/s10517-024-06047-6

Abstract

Type 2 diabetes mellitus develops due to a combination of genetic and environmental factors. C57BL/6 mice prone to obesity and leptin resistance were kept on a high-fat diet for 21 weeks. The animals showed a significant increase in fasting and postprandial glucose levels and body weight, the development of insulin resistance, and by week 18, an increase in the serum TNFα level. Metformin therapy at a dose of 250 mg/kg was effective against the background of disturbances in carbohydrate metabolism: animals showed a significant decrease in insulin resistance and TNFα level.

Keywords: C57BL/6 mice; diabetes mellitus type 2; high-fat diet; insulin resistance; metformin.

MeSH terms

Animals
Blood Glucose
Diabetes Mellitus, Type 2* / genetics
Diet, High-Fat / adverse effects
Insulin
Insulin Resistance*
Mice
Mice, Inbred C57BL
Risk Factors
Tumor Necrosis Factor-alpha / genetics

Substances

Insulin
Tumor Necrosis Factor-alpha
Blood Glucose