Isoprenoid flavonoids isolated from Sophora davidii and their activities induces apoptosis and autophagy in HT29 cells

Fitoterapia. 2024 Jun:175:105945. doi: 10.1016/j.fitote.2024.105945. Epub 2024 Apr 2.

Abstract

Four previously undescribed isoprenoid flavonoids (2-5) were isolated from Sophora davidii, along with five known analogues. The structures of the compounds were established through comprehensive analysis of spectroscopic data, including HRESIMS, 1D and 2D NMR, and absolute configurations determined by theoretical calculations, including ECD and NMR calculation. The cytotoxic effects of the isolated compounds on human HT29 colon cancer cells were evaluated using the MTT assay, compound 1 exhibited cytotoxicity against human HT29 colon cancer cells with an IC50 value of 8.39 ± 0.09 μM. Studies conducted with compound 1 in HT29 cells demonstrated that it may induce apoptosis and autophagy in HT29 by promoting the phosphorylation of P38 MAPK and inhibiting the phosphorylation of Erk MAPK.

Keywords: Apoptosis; Autophagy; HT29; Isoprenoid flavonoids; Sophora davidii.

MeSH terms

  • Antineoplastic Agents, Phytogenic* / isolation & purification
  • Antineoplastic Agents, Phytogenic* / pharmacology
  • Apoptosis* / drug effects
  • Autophagy* / drug effects
  • China
  • Flavonoids* / isolation & purification
  • Flavonoids* / pharmacology
  • HT29 Cells
  • Humans
  • Molecular Structure
  • Phosphorylation
  • Phytochemicals / isolation & purification
  • Phytochemicals / pharmacology
  • Sophora* / chemistry
  • Terpenes / isolation & purification
  • Terpenes / pharmacology
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Flavonoids
  • Antineoplastic Agents, Phytogenic
  • Phytochemicals
  • p38 Mitogen-Activated Protein Kinases
  • Terpenes