Genome-based approach to evaluate the metabolic potentials and exopolysaccharides production of Bacillus paralicheniformis CamBx3 isolated from a Chilean hot spring

Manik Prabhu Narsing Rao; Ram Nageena Singh; Rajesh K Sani; Aparna Banerjee

doi:10.3389/fmicb.2024.1377965

Genome-based approach to evaluate the metabolic potentials and exopolysaccharides production of Bacillus paralicheniformis CamBx3 isolated from a Chilean hot spring

Front Microbiol. 2024 Apr 2:15:1377965. doi: 10.3389/fmicb.2024.1377965. eCollection 2024.

Authors

Manik Prabhu Narsing Rao¹, Ram Nageena Singh^{2

3}, Rajesh K Sani^{2

3

4

5}, Aparna Banerjee¹

Affiliations

¹ Instituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, Sede Talca, Talca, Chile.
² Department of Chemical and Biological Engineering, South Dakota Mines, Rapid City, SD, United States.
³ 2-Dimensional Materials for Biofilm Engineering, Science and Technology, South Dakota Mines, Rapid City, SD, United States.
⁴ Data Driven Material Discovery Center for Bioengineering Innovation, South Dakota Mines, Rapid City, SD, United States.
⁵ BioWRAP (Bioplastics With Regenerative Agricultural Properties), Rapid City, SD, United States.

Abstract

In the present study, a thermophilic strain designated CamBx3 was isolated from the Campanario hot spring, Chile. Based on 16S rRNA gene sequence, phylogenomic, and average nucleotide identity analysis the strain CamBx3 was identified as Bacillus paralicheniformis. Genome analysis of B. paralicheniformis CamBx3 revealed the presence of genes related to heat tolerance, exopolysaccharides (EPS), dissimilatory nitrate reduction, and assimilatory sulfate reduction. The pangenome analysis of strain CamBx3 with eight Bacillus spp. resulted in 26,562 gene clusters, 7,002 shell genes, and 19,484 cloud genes. The EPS produced by B. paralicheniformis CamBx3 was extracted, partially purified, and evaluated for its functional activities. B. paralicheniformis CamBx3 EPS with concentration 5 mg mL^-1 showed an optimum 92 mM ferrous equivalent FRAP activity, while the same concentration showed a maximum 91% of Fe²⁺ chelating activity. B. paralicheniformis CamBx3 EPS (0.2 mg mL^-1) demonstrated β-glucosidase inhibition. The EPS formed a viscoelastic gel at 45°C with a maximum instantaneous viscosity of 315 Pa.s at acidic pH 5. The present study suggests that B. paralicheniformis CamBx3 could be a valuable resource for biopolymers and bioactive molecules for industrial applications.

Keywords: Baciilus paralicheniformis CamBx3; bioactive compound; exopolysaccharides; genome analysis; pangenome.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This research was funded by FONDECYT Regular, grant number 1231917 by ANID, Govt. of Chile.