Patient-Derived Orthotopic Xenograft Models for High-Grade Pediatric Brain Cancers

Jessica Buck; Hilary Hii; Raelene Endersby

doi:10.1007/978-1-0716-3858-3_5

Patient-Derived Orthotopic Xenograft Models for High-Grade Pediatric Brain Cancers

Methods Mol Biol. 2024:2806:41-53. doi: 10.1007/978-1-0716-3858-3_5.

Authors

Jessica Buck^{1

2}, Hilary Hii¹, Raelene Endersby^{3

4}

Affiliations

¹ Brain Tumor Research Program, Telethon Kids Cancer Centre, Telethon Kids Institute, Perth, WA, Australia.
² Centre for Child Health Research, University of Western Australia, Perth, WA, Australia.
³ Brain Tumor Research Program, Telethon Kids Cancer Centre, Telethon Kids Institute, Perth, WA, Australia. Raelene.endersby@telethonkids.org.au.
⁴ Centre for Child Health Research, University of Western Australia, Perth, WA, Australia. Raelene.endersby@telethonkids.org.au.

PMID: 38676795
DOI: 10.1007/978-1-0716-3858-3_5

Abstract

Patient-derived orthotopic xenograft (PDOX) mouse models are considered the gold standard for evidence-based preclinical research in pediatric neuro-oncology. This protocol describes the generation of PDOX models by intracranial implantation of human pediatric brain cancer cells into immune-deficient mice, and their continued propagation to establish cohorts of animals for preclinical research.

Keywords: Mouse models of cancer; Patient-derived xenograft; Pediatric brain cancer; Preclinical models.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain Neoplasms* / pathology
Child
Disease Models, Animal*
Heterografts
Humans
Mice
Neoplasm Grading
Xenograft Model Antitumor Assays / methods