Single-cell mapping of cell-type specific chromatin architecture in the central nervous system

Curr Opin Struct Biol. 2024 Jun:86:102824. doi: 10.1016/j.sbi.2024.102824. Epub 2024 May 8.

Abstract

Determining how chromatin is structured in the nucleus is critical to studying its role in gene regulation. Recent advances in the analysis of single-cell chromatin architecture have considerably improved our understanding of cell-type-specific chromosome conformation and nuclear architecture. In this review, we discuss the methods used for analysis of 3D chromatin conformation, including sequencing-based methods, imaging-based techniques, and computational approaches. We further review the application of these methods in the study of the role of chromatin topology in neural development and disorders.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Central Nervous System* / metabolism
  • Chromatin* / chemistry
  • Chromatin* / metabolism
  • Humans
  • Single-Cell Analysis* / methods