Synthesis of novel silver-loaded clay AgF@Hec for the prevention of dental caries in vitro

Biomed Mater. 2024 Jun 20;19(4). doi: 10.1088/1748-605X/ad51c1.

Abstract

Dental caries, a chronic infectious disease characterized by tooth mineral loss caused by plaque, is one of the major global public health problems. Silver diamine fluoride (SDF) has been proven to be a highly effective anti-caries drug due to its high bacterial inhibition and remineralization ability. However, the SDF solution is unstable, which immensely limits its clinical application. Therefore, new silver-load clay named AgF@Hec was designed by replacing the NH3with hectorite in this study. Fourier transform infrared spectroscopy and x-ray diffraction spectroscopy were employed to confirm the structure of AgF@Hec. Dynamic light scattering analysis was used to reveal the effect of different hectorite concentrations on the stability of AgF@Hec. Moreover, AgF@Hec exhibits significant remineralization and hardness recovery of the initial carious lesions. Bacteriostatic experiments also proved that it has a significant inhibitory effect onA. Viscosus, S. mutans, S. sanguinis, S. salivarius, Lactobacillus sp.and both gram-positive and gram-negative bacteria. We therefore believed that AgF@Hec should be a promising biomaterial that can be applied in the prevention of dental caries.

Keywords: biomaterial; dental caries; hectorite; sliver.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology
  • Clay* / chemistry
  • Dental Caries* / prevention & control
  • Fluorides / chemistry
  • Fluorides, Topical
  • Hardness
  • Humans
  • Materials Testing
  • Microbial Sensitivity Tests
  • Quaternary Ammonium Compounds* / chemistry
  • Quaternary Ammonium Compounds* / pharmacology
  • Silver Compounds* / chemistry
  • Silver Compounds* / pharmacology
  • Silver* / chemistry
  • Silver* / pharmacology
  • Spectroscopy, Fourier Transform Infrared
  • Streptococcus mutans / drug effects
  • Tooth Remineralization / methods
  • X-Ray Diffraction*

Substances

  • Clay
  • Silver Compounds
  • silver diamine fluoride
  • Quaternary Ammonium Compounds
  • Silver
  • Fluorides
  • Anti-Bacterial Agents
  • silver fluoride
  • Biocompatible Materials
  • Fluorides, Topical