Multiscale engineering of brain organoids for disease modeling

Adv Drug Deliv Rev. 2024 Jul:210:115344. doi: 10.1016/j.addr.2024.115344. Epub 2024 May 27.

Abstract

Brain organoids hold great potential for modeling human brain development and pathogenesis. They recapitulate certain aspects of the transcriptional trajectory, cellular diversity, tissue architecture and functions of the developing brain. In this review, we explore the engineering strategies to control the molecular-, cellular- and tissue-level inputs to achieve high-fidelity brain organoids. We review the application of brain organoids in neural disorder modeling and emerging bioengineering methods to improve data collection and feature extraction at multiscale. The integration of multiscale engineering strategies and analytical methods has significant potential to advance insight into neurological disorders and accelerate drug development.

Keywords: Brain development; Brain organoid; Disease modeling; Pluripotent stem cell; Tissue engineering.

Publication types

  • Review
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Bioengineering / methods
  • Brain* / cytology
  • Brain* / metabolism
  • Humans
  • Models, Biological
  • Nervous System Diseases / pathology
  • Organoids*
  • Tissue Engineering / methods