Shotgun metagenomic sequencing of swab samples from Japanese university campuses

Megumu Tsujimoto; Dewa A P Rasmika Dewi; Christopher E Mason; Yuh Shiwa; Haruo Suzuki

doi:10.1128/mra.00210-24

Shotgun metagenomic sequencing of swab samples from Japanese university campuses

Microbiol Resour Announc. 2024 Jul 18;13(7):e0021024. doi: 10.1128/mra.00210-24. Epub 2024 Jun 5.

Authors

Megumu Tsujimoto¹, Dewa A P Rasmika Dewi^{2

3}, Christopher E Mason^{4

5

6}, Yuh Shiwa^{7

8}, Haruo Suzuki^{1

9}

Affiliations

¹ Faculty of Environment and Information Studies, Keio University, Fujisawa, Kanagawa, Japan.
² School of Public Health and Preventive Medicine, Monash University, Melbourne, Australia.
³ School of Medicine and Health Sciences, Udayana University, Bali, Indonesia.
⁴ Department of Physiology and Biophysics, Weill Cornell Medicine, New York, New York, USA.
⁵ The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, New York, USA.
⁶ The WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, New York, USA.
⁷ Department of Molecular Microbiology, Tokyo University of Agriculture, Tokyo, Japan.
⁸ NODAI Genome Research Center, Tokyo University of Agriculture, Tokyo, Japan.
⁹ Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.

Abstract

We obtained shotgun metagenome sequences from swab samples obtained through 3-minute swabbing of different surfaces and the air within buildings at three university campuses in part of the Greater Tokyo Area in Japan. These data aid in understanding built environment microbial communities and elucidate various microbial profiles across different locations.

Keywords: DNA Data Bank of Japan; Japanese university campuses; MetaSUB international consortium; air samples (negative control); microbial community; microbiology of the built environment; shotgun metagenome sequences; surface samples (table, seat, floor); swab samples; urban microbiome.