Analysis of Toxicity of Recombinant Pneumolysin from Streptococcus pneumoniae

Bull Exp Biol Med. 2024 May;177(1):137-139. doi: 10.1007/s10517-024-06145-5. Epub 2024 Jul 4.

Abstract

We studied toxicity of recombinant Streptococcus pneumoniae pneumolysin protein in experiments on mice and its cytopathogenic effect on cultures of Vero green monkey kidney cells and human lung carcinoma A549 cells in vitro. In vivo and in vitro experiments proved the absence of compromised toxicity and direct cytopathogenic action of the recombinant protein.

Keywords: Streptococcus pneumoniae; recombinant pneumolysin; compromised toxicity; cytopathogenic impact.

MeSH terms

  • A549 Cells
  • Animals
  • Bacterial Proteins* / genetics
  • Bacterial Proteins* / toxicity
  • Chlorocebus aethiops
  • Humans
  • Mice
  • Recombinant Proteins* / genetics
  • Recombinant Proteins* / toxicity
  • Streptococcus pneumoniae* / drug effects
  • Streptolysins* / genetics
  • Streptolysins* / toxicity
  • Vero Cells

Substances

  • Streptolysins
  • plY protein, Streptococcus pneumoniae
  • Bacterial Proteins
  • Recombinant Proteins