PTX3 promotes cementum formation and cementoblast differentiation via HA/ITGB1/FAK/YAP1 signaling pathway

Bone. 2024 Oct:187:117199. doi: 10.1016/j.bone.2024.117199. Epub 2024 Jul 9.

Abstract

Cementum is a vital component of periodontium, yet its regeneration remains a challenge. Pentraxin 3 (PTX3) is a multifunctional glycoprotein involved in extracellular matrix remodeling and bone metabolism regulation. However, the role of PTX3 in cementum formation and cementoblast differentiation has not been elucidated. In this study, we initially observed an increase in PTX3 expression during cementum formation and cementoblast differentiation. Then, overexpression of PTX3 significantly enhanced the differentiation ability of cementoblasts. While conversely, PTX3 knockdown exerted an inhibitory effect. Moreover, in Ptx3-deficient mice, we found that cementum formation was hampered. Furthermore, we confirmed the presence of PTX3 within the hyaluronan (HA) matrix, thereby activating the ITGB1/FAK/YAP1 signaling pathway. Notably, inhibiting any component of this signaling pathway partially reduced the ability of PTX3 to promote cementoblast differentiation. In conclusion, our study indicated that PTX3 promotes cementum formation and cementoblast differentiation, which is partially dependent on the HA/ITGB1/FAK/YAP1 signaling pathway. This research will contribute to our understanding of cementum regeneration after destruction.

Keywords: Cementoblast; Dental cementum; Hyaluronan; PTX3; Signal transduction; YAP1.

MeSH terms

  • Adaptor Proteins, Signal Transducing* / metabolism
  • Animals
  • C-Reactive Protein / metabolism
  • Cell Differentiation*
  • Cementogenesis
  • Dental Cementum* / metabolism
  • Focal Adhesion Kinase 1 / genetics
  • Focal Adhesion Kinase 1 / metabolism
  • Integrin beta1 / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Serum Amyloid P-Component / genetics
  • Serum Amyloid P-Component / metabolism
  • Signal Transduction*
  • YAP-Signaling Proteins* / metabolism

Substances

  • YAP-Signaling Proteins
  • Adaptor Proteins, Signal Transducing
  • Yap1 protein, mouse
  • C-Reactive Protein
  • Integrin beta1
  • PTX3 protein
  • Serum Amyloid P-Component
  • Focal Adhesion Kinase 1