Induction of the PERK-eIF2α-ATF4 Pathway in M1 Macrophages under Endoplasmic Reticulum Stress

Dokl Biochem Biophys. 2024 Aug;517(1):264-268. doi: 10.1134/S1607672924600301. Epub 2024 Jul 13.

Abstract

Translation inhibition can activate two cell death pathways. The first pathway is activated by translational aberrations, the second by endoplasmic reticulum (ER) stress. In this work, the effect of ribosome-inactivating protein type II (RIP-II) viscumin on M1 macrophages derived from the THP-1 cell line was investigated. The number of modified ribosomes was evaluated by real-time PCR. Transcriptome analysis revealed that viscumin induces the ER stress activated by the PERK sensor.

Keywords: ER stress; M1 macrophages; PERK-eIF2α-ATF4 pathway; THP-1; ribosome-inactivating protein; viscumin.

MeSH terms

  • Activating Transcription Factor 4* / genetics
  • Activating Transcription Factor 4* / metabolism
  • Endoplasmic Reticulum Stress* / drug effects
  • Eukaryotic Initiation Factor-2* / metabolism
  • Humans
  • Macrophages* / drug effects
  • Macrophages* / metabolism
  • Signal Transduction*
  • THP-1 Cells
  • eIF-2 Kinase* / genetics
  • eIF-2 Kinase* / metabolism

Substances

  • Eukaryotic Initiation Factor-2
  • eIF-2 Kinase
  • Activating Transcription Factor 4
  • EIF2AK3 protein, human
  • ATF4 protein, human