A getah virus-like-particle vaccine provides complete protection from viremia and arthritis in wild-type mice

Vaccine. 2024 Nov 14;42(25):126136. doi: 10.1016/j.vaccine.2024.07.037. Epub 2024 Jul 14.

Abstract

Getah virus (GETV) is an emerging mosquito-borne virus with economic impact on the livestock industry in East Asia. In this study, we successfully produced GETV virus-like particles (VLPs) in insect cells using the baculovirus expression vector system. We show that the GETV envelope glycoproteins were successfully expressed at the surface of the insect cell and were glycosylated. VLPs were isolated from the culture fluid as enveloped particles of 60-80 nm in diameter. Two 1 µg vaccinations with this GETV VLP vaccine, without adjuvant, generated neutralizing antibody responses and protected wild-type C57/BL6 mice against GETV viremia and arthritic disease. The GETV VLP vaccine may find application as a horse and/or pig vaccine in the future.

Keywords: Baculovirus; Getah virus; Mouse model; Vaccine; Virus-like-particle.

MeSH terms

  • Alphavirus Infections / immunology
  • Alphavirus Infections / prevention & control
  • Alphavirus* / genetics
  • Alphavirus* / immunology
  • Animals
  • Antibodies, Neutralizing* / blood
  • Antibodies, Neutralizing* / immunology
  • Antibodies, Viral* / blood
  • Antibodies, Viral* / immunology
  • Arthritis* / immunology
  • Arthritis* / prevention & control
  • Baculoviridae / genetics
  • Baculoviridae / immunology
  • Female
  • Mice
  • Mice, Inbred C57BL*
  • Sf9 Cells
  • Vaccines, Virus-Like Particle* / administration & dosage
  • Vaccines, Virus-Like Particle* / immunology
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology
  • Viremia* / immunology
  • Viremia* / prevention & control

Substances

  • Vaccines, Virus-Like Particle
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Viral Vaccines
  • Viral Envelope Proteins