Genomic profiling of lymph node and distant metastases from papillary and poorly differentiated thyroid carcinomas

Endocrine. 2024 Nov;86(2):505-509. doi: 10.1007/s12020-024-03968-0. Epub 2024 Jul 19.

Abstract

Purpose: To perform a molecular profiling of the metastases from papillary thyroid carcinomas (PTCs) and poorly differentiated thyroid carcinomas (PDTCs).

Methods: We retrieved and analyzed the molecular and clinical features of 136 metastases from PTCs and 35 metastases from PDTCs subjected to targeted DNA sequencing, from cBioPortal. The clinicopathological data included the number and location of the metastases, and genomic data included mutations, translocations, copy number alterations and fraction of the genome altered (FGA).

Results: Bone metastases from PTCs had a lower frequency of BRAF mutations than the lymph node metastases (LNMs) (43% vs 88%, p < 0.01), and a higher frequency of RBM10 and NRAS mutations than the LNMs (21% vs 3% for both, p < 0.05). The FGA of the bone metastases was higher than the FGA of the lung metastases (5.6% vs 1.3%, p < 0.05). The frequency of RET translocations was higher in the lung metastases from PTCs than the LNMs (15% vs 3%, p < 0.05). The LNMs from PTC patients harboring 4 or more distant metastases (DMs) had a higher frequency of TERT promoter mutations than the LNMs from patients harboring less than 4 DMs (96% vs 65%, p < 0.001). SDHA gene amplifications were enriched in the bone metastases from PDTCs and absent in the LNMs (38% vs 0%, p < 0.05).

Conclusion: Metastases from PTCs and PDTCs harbor clinically relevant alterations affecting distinct body locations, such as NRAS and RBM10 mutations, RET translocations and SDHA amplifications that may be explored therapeutically.

Keywords: Copy number alterations; DNA sequencing; Distant metastasis; Papillary thyroid carcinomas; Poorly differentiated thyroid carcinomas; Somatic mutations.

MeSH terms

  • Adult
  • Aged
  • Bone Neoplasms / genetics
  • Bone Neoplasms / secondary
  • Carcinoma, Papillary / genetics
  • Carcinoma, Papillary / pathology
  • DNA Copy Number Variations
  • Female
  • GTP Phosphohydrolases / genetics
  • Humans
  • Lung Neoplasms / genetics
  • Lung Neoplasms / pathology
  • Lymph Nodes / pathology
  • Lymphatic Metastasis* / genetics
  • Lymphatic Metastasis* / pathology
  • Male
  • Membrane Proteins / genetics
  • Middle Aged
  • Mutation*
  • Proto-Oncogene Proteins B-raf / genetics
  • Thyroid Cancer, Papillary* / genetics
  • Thyroid Cancer, Papillary* / pathology
  • Thyroid Neoplasms* / genetics
  • Thyroid Neoplasms* / pathology

Substances

  • NRAS protein, human
  • GTP Phosphohydrolases
  • Membrane Proteins
  • Proto-Oncogene Proteins B-raf
  • BRAF protein, human