Molecular detection of Usutu virus in pools of Culex pipiens mosquitoes in Greece

Acta Trop. 2024 Oct:258:107330. doi: 10.1016/j.actatropica.2024.107330. Epub 2024 Jul 21.

Abstract

Usutu virus (USUV) is a mosquito-borne flavivirus originating from Africa, that belongs to the Japanese encephalitis virus (JEV) complex. In nature, USUV involves Culex spp. mosquitoes acting as vectors and birds as amplifying hosts. The virus has recently spread in Europe and is considered an emerging human pathogen. This is the first research study performed in Greece revealing the presence and circulation of USUV in Culex spp. mosquito populations. Out of the 1,500 mosquito pools tested with real-time RT-PCR, four (Roesch et al., 2019) were positive for USUV. All four pools were collected from the region of Central Macedonia, Northern Greece.

Keywords: Climate change; Flavivirus; Sequencing analysis; Vector.

MeSH terms

  • Animals
  • Culex* / virology
  • Flavivirus Infections / transmission
  • Flavivirus Infections / veterinary
  • Flavivirus Infections / virology
  • Flavivirus* / classification
  • Flavivirus* / genetics
  • Flavivirus* / isolation & purification
  • Greece
  • Mosquito Vectors* / virology
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Real-Time Polymerase Chain Reaction

Substances

  • RNA, Viral

Supplementary concepts

  • Usutu virus
  • Culex pipiens