Variation to biology: optimizing functional analysis of cancer risk variants

J Natl Cancer Inst. 2024 Dec 1;116(12):1882-1889. doi: 10.1093/jnci/djae173.

Abstract

Research conducted over the past 15+ years has identified hundreds of common germline genetic variants associated with cancer risk, but understanding the biological impact of these primarily non-protein coding variants has been challenging. The National Cancer Institute sought to better understand and address those challenges by requesting input from the scientific community via a survey and a 2-day virtual meeting, which focused on discussions among participants. Here, we discuss challenges identified through the survey as important to advancing functional analysis of common cancer risk variants: 1) When is a variant truly characterized; 2) Developing and standardizing databases and computational tools; 3) Optimization and implementation of high-throughput assays; 4) Use of model organisms for understanding variant function; 5) Diversity in data and assays; and 6) Creating and improving large multidisciplinary collaborations. We define these 6 challenges, describe how success in addressing them may look, propose potential solutions, and note issues that span all the challenges. Implementation of these ideas could help develop a framework for methodically analyzing common cancer risk variants to understand their function and make effective and efficient use of the wealth of existing genomic association data.

MeSH terms

  • Computational Biology
  • Databases, Genetic
  • Genetic Predisposition to Disease*
  • Genetic Variation
  • Germ-Line Mutation
  • Humans
  • National Cancer Institute (U.S.)
  • Neoplasms* / genetics
  • Risk Factors
  • United States / epidemiology