Lipidomic study of kidney in a mouse model with urine flow obstruction

Divyavani Gowda; Md Abdul Masum; Siddabasave Gowda B Gowda; Chandra Shekhar; Md Zahir Uddin Rubel; Shunnosuke Kira; Osamu Ichii; Yasuhiro Kon; Hitoshi Chiba; Shu-Ping Hui

doi:10.1038/s41598-024-68270-5

Lipidomic study of kidney in a mouse model with urine flow obstruction

Sci Rep. 2024 Aug 5;14(1):18042. doi: 10.1038/s41598-024-68270-5.

Authors

Divyavani Gowda^#¹, Md Abdul Masum^#^{2

3}, Siddabasave Gowda B Gowda^{1

4}, Chandra Shekhar^{1

5}, Md Zahir Uddin Rubel⁶, Shunnosuke Kira⁶, Osamu Ichii^{6

7}, Yasuhiro Kon⁶, Hitoshi Chiba⁸, Shu-Ping Hui⁹

Affiliations

¹ Faculty of Health Sciences, Hokkaido University, Kita-12 Nishi-5, Kita-ku, Sapporo, 060-0812, Japan.
² Department of Anatomy, Histology and Physiology, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh. masum@sau.edu.bd.
³ Laboratory of Anatomy, Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan. masum@sau.edu.bd.
⁴ Graduate School of Global Food Resources, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo, 060-0809, Japan.
⁵ Departments of Physiology, Medicine, Molecular Biology Immunology and Biochemistry, and Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, TN, 38163, USA.
⁶ Laboratory of Anatomy, Department of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
⁷ Laboratory of Agrobiomedical Science, Faculty of Agriculture, Hokkaido University, Sapporo, 060-0809, Japan.
⁸ Department of Nutrition, Sapporo University of Health Sciences, Nakanuma, Nishi-4-3-1-15, Higashi-ku, Sapporo, 007-0894, Japan.
⁹ Faculty of Health Sciences, Hokkaido University, Kita-12 Nishi-5, Kita-ku, Sapporo, 060-0812, Japan. keino@hs.hokudai.ac.jp.

^# Contributed equally.

Abstract

Obstructed urine flow is known to cause structural and functional kidney damage leading to renal fibrosis. However, limited information is available on the change in kidney lipids during urinary tract obstruction. In this study, we investigated the change in lipidome in a mouse model with unilateral ureteral obstruction (UUO). The establishment of the UUO model was confirmed by histopathological examination using transmission electron microscopy. Untargeted liquid chromatography/mass spectrometry was carried out over a time course of 4 and 7 days. Compared to the sham control, the UUO kidney at 7 days showed dilatation of the renal tubule with loss of brush borders and thickening of the capillary endothelium. In the kidney lipidomes obtained from the UUO 7 days group compared to the control, a significant decrease of ceramide, sphingomyelin, phosphatidylcholine, lysophospholipids, and phosphatidylethanolamine was observed, whereas cholesteryl esters, free fatty acids, phosphatidylglycerol, and cardiolipins were significantly increased. The present study revealed the disturbed lipid metabolism in the UUO model, which may provide a clue to potential lipid pathways and therapeutic targets for the early stage of renal fibrosis.

Keywords: Kidney injury; Lipid; Liquid chromatography; Mass spectrometry; Renal tubulointerstitial lesion; Unilateral ureteral obstruction.

MeSH terms

Animals
Disease Models, Animal*
Fibrosis
Kidney* / metabolism
Kidney* / pathology
Lipid Metabolism*
Lipidomics* / methods
Male
Mice
Mice, Inbred C57BL
Ureteral Obstruction* / metabolism
Ureteral Obstruction* / pathology

Abstract

MeSH terms

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