IL10 and CXCL10 mRNA expression in food protein-induced enterocolitis syndrome

Cytokine. 2024 Oct:182:156720. doi: 10.1016/j.cyto.2024.156720. Epub 2024 Aug 11.

Abstract

Background: Food protein-induced enterocolitis syndrome (FPIES) is a rare non-IgE-mediated food allergy that mainly impacts babies and 7toddlers. The exact mechanism of FPIES is not completely understood. By studying the expression of IL-10 and CXCL10 in pediatric FPIES patients, researchers can gain insights into the immune mechanisms underlying this disorder.

Methods: Peripheral venous blood was collected and subsequently stabilized with RNA pro. Total RNA was extracted and mRNA levels of CXCL10 and IL-10 was determined with real time PCR.

Results: Children with FPIES had significantly higher values than the healthy control group (HC) for CXCL10 while FPIES had a significant lower values than the control group for IL-10.

Conclusions: Our results show a high production of CXCL10 and a concomitant reduced production of IL-10 in FPIES subjects who have not yet reached tolerance. These data may represent a molecular diagnostic marker for FPIES.

Keywords: CXCL10; Children; Food protein–induced enterocolitis syndrome (FPIES); IL10.

MeSH terms

  • Case-Control Studies
  • Chemokine CXCL10* / blood
  • Chemokine CXCL10* / genetics
  • Child
  • Child, Preschool
  • Enterocolitis* / genetics
  • Enterocolitis* / immunology
  • Female
  • Food Hypersensitivity*
  • Humans
  • Infant
  • Interleukin-10* / blood
  • Interleukin-10* / genetics
  • Male
  • RNA, Messenger* / genetics
  • RNA, Messenger* / metabolism
  • Syndrome

Substances

  • Interleukin-10
  • Chemokine CXCL10
  • RNA, Messenger
  • CXCL10 protein, human
  • IL10 protein, human