Validation of a modified enrichment broth for efficient screening of group B Streptococcus in pregnant women

Daiki Tanno; Kyoichi Saito; Yasuaki Tomii; Yukari Nakatsuka; Kohei Uechi; Kazutaka Ohashi; Yukio Yamadera; Atsuko Hata; Masahiro Toyokawa; Hiroki Shimura

doi:10.1016/j.jmii.2024.07.015

Validation of a modified enrichment broth for efficient screening of group B Streptococcus in pregnant women

J Microbiol Immunol Infect. 2024 Dec;57(6):957-960. doi: 10.1016/j.jmii.2024.07.015. Epub 2024 Aug 3.

Authors

Affiliations

¹ Department of Clinical Laboratory Sciences, School of Health Sciences, Fukushima Medical University, Fukushima, Japan; Department of Clinical Laboratory, Fukushima Medical University Hospital, Fukushima, Japan; Department of Laboratory Medicine, School of Medicine, Fukushima Medical University, Fukushima, Japan. Electronic address: dtanno@fmu.ac.jp.
² Department of Clinical Laboratory, Fukushima Medical University Hospital, Fukushima, Japan; Department of Laboratory Medicine, School of Medicine, Fukushima Medical University, Fukushima, Japan.
³ Department of Clinical Laboratory, Aiiku Hospital, Tokyo, Japan.
⁴ Department of Laboratory Medicine, Medical Research Institute KITANO HOSPITAL, PIIF Tazuke-kofukai, Osaka, Japan.
⁵ Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital, Okinawa, Japan.
⁶ Department of Clinical Laboratory, Fukushima Medical University Hospital, Fukushima, Japan.
⁷ Department of Pediatrics, Division of Infectious Diseases, Medical Research Institute KITANO HOSPITAL, PIIF Tazuke-kofukai, Osaka, Japan.
⁸ Department of Clinical Laboratory Sciences, School of Health Sciences, Fukushima Medical University, Fukushima, Japan; Department of Clinical Laboratory, Fukushima Medical University Hospital, Fukushima, Japan; Department of Laboratory Medicine, School of Medicine, Fukushima Medical University, Fukushima, Japan.

PMID: 39142909
DOI: 10.1016/j.jmii.2024.07.015

Abstract

We validated a modified enrichment broth that changes its color when group B Streptococcus (GBS) grows. No GBS was detected in any of the non-yellow samples. Thus, the non-yellow samples were considered GBS-negative without conducting further examinations, potentially reducing medical costs and workload.

Keywords: GBS screening in pregnant women; Selective enrichment broth; Streptococcus agalactiae.

Publication types

Validation Study

MeSH terms

Bacteriological Techniques / methods
Culture Media*
Female
Humans
Mass Screening / methods
Pregnancy
Pregnancy Complications, Infectious* / diagnosis
Pregnancy Complications, Infectious* / microbiology
Streptococcal Infections* / diagnosis
Streptococcal Infections* / microbiology
Streptococcus agalactiae* / isolation & purification

Substances

Culture Media