Protocol for preparing mammalian skin samples encompassing hair follicles for spatial transcriptomics

STAR Protoc. 2024 Sep 20;5(3):103254. doi: 10.1016/j.xpro.2024.103254. Epub 2024 Aug 14.

Abstract

Spatial transcriptomics enables a single-cell resolution view of gene expression patterns in tissues, providing insight into their biological functions. However, applying this approach to the skin presents inherent challenges. Here, we present a protocol for preparing mammalian skin samples encompassing hair follicles for spatial transcriptomics. We describe steps for sample preparation, embedding, acquisition of frozen slices, RNA quality control, tissue mounting, fixation, staining, and imaging. We then detail procedures for permeabilization, reverse transcription, and cDNA collection. For complete details on the use and execution of this protocol, please refer to Chen et al.1.

Keywords: Genetics; Genomics; Model Organisms; Molecular Biology.

MeSH terms

  • Animals
  • Gene Expression Profiling* / methods
  • Hair Follicle* / cytology
  • Hair Follicle* / metabolism
  • Humans
  • Mammals / genetics
  • Mice
  • Skin* / metabolism
  • Tissue Fixation / methods
  • Transcriptome* / genetics