Abstract
Phytochemical study on the methanol extract of the leaves of Phyllanthus reticulatus led to the isolation of two new compounds, phyllanretiosides A (1) and B (2) together with ten known ones (3-12). Their chemical structures were determined by HR-ESI-MS, NMR, and ECD spectra in comparison with the literature. Three ellagitannins, phyllanretioside A (1), corilagin (3), and phyllanthusiin C (4) inhibited lipopolysaccharide(LPS)-induced nitric oxide production in RAW 264.7 cells with IC50 values of 63.2, 83.2, and 5.6 μM, respectively. In addition, compounds 1, 3, and 4 exhibited significantly antimicrobial activity (MICs: 16-128 μg/mL) towards some of seven microbial strains.
Keywords:
Antimicrobial activity; Nitric oxide inhibition activity; Phyllanreticoside A; Phyllanreticoside B; Phyllanthus reticulatus.
© 2024 Wiley-VHCA AG, Zurich, Switzerland.
MeSH terms
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Animals
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology
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Anti-Infective Agents / chemistry
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Anti-Infective Agents / isolation & purification
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Anti-Infective Agents / pharmacology
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Hydrolyzable Tannins / chemistry
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Hydrolyzable Tannins / isolation & purification
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Hydrolyzable Tannins / pharmacology
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Lipopolysaccharides* / antagonists & inhibitors
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Lipopolysaccharides* / pharmacology
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Mice
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Microbial Sensitivity Tests*
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Molecular Conformation
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Molecular Structure
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Nitric Oxide* / antagonists & inhibitors
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Nitric Oxide* / biosynthesis
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Nitric Oxide* / metabolism
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Phyllanthus* / chemistry
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology
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Plant Leaves / chemistry
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RAW 264.7 Cells
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Structure-Activity Relationship
Substances
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Nitric Oxide
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Lipopolysaccharides
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Hydrolyzable Tannins
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Anti-Bacterial Agents
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Anti-Infective Agents
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Plant Extracts