Objective: To investigate the expression level, clinical significance and function of circular RNAs (circRNAs) circ_0073585 in the bone marrow of patients with acute myeloid leukemia (AML).
Methods: The expression levels of circ_0073585 in bone marrow samples of 106 newly diagnosed AML patients and 38 controls were detected by real-time quantitative PCR (RQ-PCR). The differences were compared between the two groups and their clinical significance was analyzed. The diagnostic value of circ_0073585 expression for AML was evaluated by receiver operating characteristic curve(ROC). THP-1 cells with lentivirus overexpressing circ_0073585 vector and empty vector were divided into two groups: circ_0073585-THP-1 and NC-THP-1 group. CCK-8 assay and flow cytometry were used to study the effects of circ_0073585 on THP-1 cell proliferation, survival, apoptosis and drug sensitivity.
Results: Compared with the controls, the expression level of circ_0073585 in the bone marrow of AML patients was significantly reduced (P < 0.001). There was a statistically significant difference between the high and low expression groups of circ_0073585 in the white blood cell count, platelet count (P < 0.01) and chromosome risk (P < 0.05). Compared with NC-THP-1 cells, the proliferation and viability of circ_0073585-THP-1 cells were weakened (P < 0.01), the apoptosis rate was increased (P < 0.01), and the sensitivity to homoharringtonine (P < 0.05) and daunorubicin hydrochloride (P < 0.001) was increased.
Conclusion: The expression level of circ_0073585 is decreased in AML patients. Overexpression of circ_0073585 can inhibit the proliferation and viability of leukemic cells, promote apoptosis, and increase sensitivity of leukemia cells to chemotherapy drugs.
题目: circ_0073585在急性髓系白血病中的表达及功能研究.
目的: 研究环状RNA（circular RNA, circRNA）circ_0073585在急性髓系白血病（AML）患者骨髓中的表达水平、临床意义及其在白血病细胞中的功能。.
方法: 采用实时定量PCR（RQ-PCR）检测106例初诊AML患者及38例对照者骨髓标本中circ_0073585的表达水平，比较两组的表达差异并分析其临床意义。根据受试者工作特征（ROC）曲线来评估circ_0073585的表达对AML的诊断价值。将过表达circ_0073585载体及空载体的慢病毒感染的THP-1细胞分为circ_0073585-THP-1和NC-THP-1两组，通过CCK-8法、流式细胞术分析circ_0073585对THP-1细胞增殖、生存、凋亡及药物敏感性的影响。.
结果: 与对照组相比，circ_0073585在AML患者骨髓中的表达水平显著降低（P < 0.001）。circ_0073585高、低表达组在白细胞计数（P < 0.05）、血小板计数（P < 0.01）及染色体危险程度（P < 0.05）方面差异有统计意义。与NC-THP-1细胞相比，circ_0073585-THP-1细胞增殖及生存能力减弱（P < 0.01）、凋亡率增加（P < 0.01），对高三尖杉酯碱（P < 0.05）及柔红霉素（P < 0.001）的敏感性增加。.
结论: circ_0073585在AML患者中表达水平下降，过表达circ_0073585可抑制白血病细胞的增殖能力、生存能力，促进凋亡，并提高白血病细胞对化疗药物的敏感性。.
Keywords: circular RNA; circ_0073585; acute myeloid leukemia.