Bimetal-Biligand Frameworks for Spatiotemporal Nitric Oxide-Enhanced Sono-Immunotherapy

Adv Mater. 2024 Nov;36(45):e2408242. doi: 10.1002/adma.202408242. Epub 2024 Sep 3.

Abstract

Sonodynamic therapy can trigger immunogenic cell death to augment immunotherapy, benefiting from its superior spatiotemporal selectivity and non-invasiveness. However, the practical applications of sonosensitizers are hindered by their low efficacy in killing cancer cells and activating immune responses. Here, two US Food and Drug Administration-approved drug ligands (ferricyanide and nitroprusside) and two types of metals (copper/iron) are selected to construct a bimetal-biligand framework (Cu[PBA-NO]). Through elaborate regulation of multiple metal/ligand coordination, the systemically administered Cu[PBA-NO] nanoagent shows sono-catalytic and NO release ability under ultrasound irradiation, which can be used for effective sono-immunotherapy. Moreover, Cu[PBA-NO] can downregulate intracellular glutathione levels that would destroy intracellular redox homeostasis and facilitate reactive oxygen species accumulation. The released tumor-associated antigens subsequently facilitate dendritic cell maturation within the tumor-draining lymph node, effectively initiating a T cell-mediated immune response and thereby bolstering the capacity to identify and combat cancer cells. This study paves a new avenue for the efficient cancer sono-immunotherapy.

Keywords: bimetal‐biligand frameworks; cancer treatment; immunotherapy; nitric oxide; sonodynamic therapy.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Copper / chemistry
  • Dendritic Cells / drug effects
  • Glutathione / chemistry
  • Glutathione / metabolism
  • Humans
  • Immunotherapy*
  • Ligands
  • Metal-Organic Frameworks* / chemistry
  • Metal-Organic Frameworks* / pharmacology
  • Mice
  • Neoplasms / drug therapy
  • Neoplasms / therapy
  • Nitric Oxide* / metabolism
  • Reactive Oxygen Species / metabolism
  • Ultrasonic Therapy / methods

Substances

  • Nitric Oxide
  • Metal-Organic Frameworks
  • Copper
  • Reactive Oxygen Species
  • Ligands
  • Glutathione